ANION EXCHANGE CHROMATOGRAPHY OF ACIDS OF THE CITRIC ACID CYCLE
نویسندگان
چکیده
منابع مشابه
Anion exchange chromatography of acids of the citric acid cycle.
The demonstration of the accumulation of citrate in vivo in the presence of fluoroacetate and the specific modification of this effect produced in various organs by the injection of malonate (14) have increased the desirability of isolation and quantitative determination of the acids of the Krebs cycle and related substances. Further, proposed studies involving the use of radioactive tracers re...
متن کاملAnalysis of Sialic Acids Using High-Performance Anion-Exchange Chromatography
INTRODUCTION Sialic acids comprise a large family of Nand O-substituted neuraminic acids. The amino group of neuraminic acid is linked to either an N-acetyl or N-glycolyl group, which yields N-acetylneuraminic acid (Neu5Ac) and N-glycolylneuraminic acid (Neu5Gc), respectively. The hydroxyls of both sialic acids are often modified. The biosynthesis, function, and occurrence of sialic acids have ...
متن کاملAcid-catalyzed lactonization of alpha2,8-linked oligo/polysialic acids studied by high performance anion-exchange chromatography.
Recent studies from many laboratories revealed remarkable structural, distributional, and functional diversities of oligo/polysialic acids (OSA/PSA) that exist in organisms ranging from bacteria to man. These diversities are further complicated by the fact that OSA/PSA spontaneously form lactones under even mildly acidic conditions. By using high performance anion-exchange chromatography (HPAEC...
متن کاملAnion-Exchange Chromatography of Phosphopeptides: Weak Anion Exchange versus Strong Anion Exchange and Anion-Exchange Chromatography versus Electrostatic Repulsion–Hydrophilic Interaction Chromatography
Most phosphoproteomics experiments rely on prefractionation of tryptic digests before online liquid chromatography-mass spectrometry. This study compares the potential and limitations of electrostatic repulsion-hydrophilic interaction chromatography (ERLIC) and anion-exchange chromatography (AEX). At a pH higher than 5, phosphopeptides have two negative charges per residue and are well-retained...
متن کاملOptimization of Dynamic Binding Capacity of Anion Exchange Chromatography Media for Recombinant Erythropoietin Purification
Background:The dynamic binding capacity (DBC) of a chromatography matrix in protein purification is the amount of the total protein absorbed into the matrix, before occurrence of a significant break in the breakthrough curve. Optimization of the process criteria for maximum DBC avoids extra process scale-up and reduces ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1952
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)52403-6